Tetrazolium salts are an important class of leuco dyes that have found a wide range of applications. They are unique among the synthetic leuco dyes in that the colored form of the dye is the reduced form rather than the oxidized form. There are at least three extensive reviews on tetrazolium salts and the corresponding formazan dyes1-3 and one textbook.96 This chapter will describe some recent developments as well as some of the material covered in these reviews.
Tetrazolium salts to a large extent are prepared from the corresponding formazans and to a lesser extent from tetrazoles. Therefore, synthetic methods for both formazans and tetrazoles will be discussed. Also discussed will be some properties of formazan dyes that influence the choice of the tetrazolium salt for any particular application.