NOMENCLATURE AND RING NUMBERING SYSTEM

Tetrazolium salts are derivatives of the 1H-tetrazole (1) or 2H-tetrazole (2). The CA numbering systems for tetrazoliums and formazans are shown in 3 and 4. It should be noted that the carbon atoms in 3 and 4 are

DANIEL S. DANIEL • Clinical Chemistry Research and Development, Johnson & Johnson Clinical Diagnostics, Rochester, New York 14650-2113.

Chemistry and Applications of Leuco Dyes, edited by Muthyala. Plenum Press, New York, 1997.

NOMENCLATURE AND RING NUMBERING SYSTEM

numbered 3 and 5, respectively. For example, where R and R2 are phenyls and R3 is methyl, 3 is named 3-methyl — 1,5-diphenylformazan, while the tetrazolium salt 4 is named 5-methyl-2,3-diphenyl-2#-tetrazolium. Some authors refer to the more common tetrazolium salts by trivial names, usually based on the color of the formazan obtained through reduction of the salt. These names have seldom been applied consistently and have caused some confusion.

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