Most analytical uses of tetrazolium salts utilize their reducibility. The ability of the resulting formazan to coordinate to metal ions, causing a characteristic shift in the spectrum, is often utilized to determine the metal ions or to enhance the sensitivity of the detection. Determination of Reducing Substances

A number of reducing agents have been determined directly using tetrazolium salts. Thus, the analyses of reducing sugars,423,424 hydraz — ides, 425 sulfides and thiols,426 ascorbic acid,427,428 formaldehyde,429 L — dopa430 among others have been reported. The method is extended to the analysis of some drugs,431 — 433 antibiotics,434 and pesticides.435 Proteins and nucleic acids438 can be determined indirectly. Enzymatic methods will be discussed in Section 7.6.2.

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